Nerve growth factor-stimulated mitogen-activated protein kinase activity is not necessary for neurite outgrowth of chick dorsal root ganglion sensory and sympathetic neurons

J Neurosci Res. 1996 Dec 15;46(6):720-6. doi: 10.1002/(SICI)1097-4547(19961215)46:6<720::AID-JNR8>3.0.CO;2-H.


Nerve growth factor (NGF)-stimulated neurite outgrowth in the rat PC12 tumor cell line recently has been shown to depend on the activation of the mitogen-activated protein (MAP) kinase kinase 1 (MEK1) (Pang et al.: J Biol Chem 270:13585-13588, 1995). In this study we have analyzed whether or not function of the MAP kinase pathway is necessary for NGF-stimulated neurite outgrowth in two subtypes of primary neurons derived from the embryonic chick peripheral nervous system (PNS). Treatment of p21ras-dependent dorsal root ganglion (DRG) sensory neurons (E9) with the MEK1 inhibitor PD98059 at concentrations up to 100 microM did not prevent NGF-stimulated neurite outgrowth. At this concentration NGF-stimulated tyrosine phosphorylation of MAP kinase p42 as well as MAP kinase activity both were decreased by approximately 80%. Essentially the same results were obtained with p21ras-independent sympathetic neurons (E12). We conclude that, in contrast to the PC12 tumor cell line, NGF-stimulated MAP kinase activity is not necessary for neurite outgrowth of DRG sensory and sympathetic neurons derived from the chick PNS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured / drug effects
  • Cells, Cultured / physiology
  • Chick Embryo
  • Enzyme Inhibitors / pharmacology
  • Flavonoids / pharmacology
  • Ganglia, Spinal / cytology*
  • Ganglia, Sympathetic / cytology*
  • MAP Kinase Kinase 1
  • Male
  • Mice
  • Mitogen-Activated Protein Kinase Kinases
  • Mitogens / pharmacology
  • Nerve Growth Factors / pharmacology
  • Neurites / drug effects
  • Neurites / physiology*
  • Neurons, Afferent / cytology
  • Neurons, Afferent / enzymology*
  • Neurons, Afferent / ultrastructure
  • Peripheral Nerves / cytology
  • Phosphorylation
  • Protein Kinase C / metabolism*
  • Protein Kinase Inhibitors
  • Protein Kinases / metabolism
  • Protein Serine-Threonine Kinases / metabolism
  • Protein-Tyrosine Kinases / metabolism
  • Signal Transduction / physiology
  • Tyrosine / metabolism


  • Enzyme Inhibitors
  • Flavonoids
  • Mitogens
  • Nerve Growth Factors
  • Protein Kinase Inhibitors
  • Tyrosine
  • Protein Kinases
  • Protein-Tyrosine Kinases
  • Protein Serine-Threonine Kinases
  • Protein Kinase C
  • MAP Kinase Kinase 1
  • Map2k1 protein, mouse
  • Mitogen-Activated Protein Kinase Kinases
  • 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one