Eomesodermin, a key early gene in Xenopus mesoderm differentiation

Cell. 1996 Dec 13;87(6):989-1000. doi: 10.1016/s0092-8674(00)81794-8.

Abstract

Eomesodermin (Eomes) is a novel Xenopus T-domain gene. In normal development, it is expressed in mesodermal cells in a ventral-to-dorsal gradient of increasing concentration. It reaches its peak expression 1-2 hr before any other known panmesodermal gene. It is strongly inducible by normal vegetal cells and by mesoderm-inducing factors. Ectopic expression of Eomes in animal caps induces the transcription of nearly all mesodermal genes in a concentration-dependent way. Overexpression of Eomes dorsalizes ventral mesoderm, inducing gsc and changing cell fate to muscle and notochord. Blocking the function of Eomes causes gastrulation arrest and defective mesoderm-dependent gene activation. We propose that Eomes fulfills an essential function in initiating mesoderm differentiation and in determining mesodermal cell fate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Activins
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Communication / genetics
  • Cell Differentiation / genetics
  • DNA-Binding Proteins / physiology
  • Embryonic Induction / physiology*
  • Fetal Proteins / physiology
  • Gene Expression Regulation, Developmental / drug effects
  • Gene Expression Regulation, Developmental / genetics
  • Genes, Immediate-Early / drug effects
  • Genes, Immediate-Early / physiology
  • Growth Substances / pharmacology
  • Inhibins / pharmacology
  • Mesoderm / cytology
  • Mesoderm / physiology*
  • Molecular Sequence Data
  • Mutagenesis / physiology
  • T-Box Domain Proteins*
  • Time Factors
  • Transcription Factors / genetics*
  • Transcription, Genetic / physiology
  • Transcriptional Activation
  • Xenopus / genetics*
  • Xenopus Proteins*

Substances

  • DNA-Binding Proteins
  • EOMES protein, Xenopus
  • Fetal Proteins
  • Growth Substances
  • T-Box Domain Proteins
  • Transcription Factors
  • Xenopus Proteins
  • Activins
  • Inhibins
  • Brachyury protein

Associated data

  • GENBANK/U75996