Transcription activation at class II CAP-dependent promoters: two interactions between CAP and RNA polymerase

Cell. 1996 Dec 13;87(6):1123-34. doi: 10.1016/s0092-8674(00)81806-1.


At Class II catabolite activator protein (CAP)-dependent promoters, CAP activates transcription from a DNA site overlapping the DNA site for RNA polymerase. We show that transcription activation at Class II CAP-dependent promoters requires not only the previously characterized interaction between an activating region of CAP and the RNA polymerase alpha subunit C-terminal domain, but also an interaction between a second, promoter-class-specific activating region of CAP and the RNA polymerase alpha subunit N-terminal domain. We further show that the two interactions affect different steps in transcription initiation. Transcription activation at Class II CAP-dependent promoters provides a paradigm for understanding how an activator can make multiple interactions with the transcription machinery, each interaction being responsible for a specific mechanistic consequence.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acids / chemistry
  • Binding Sites / genetics
  • Cyclic AMP Receptor Protein / chemistry
  • Cyclic AMP Receptor Protein / genetics*
  • Cyclic AMP Receptor Protein / metabolism
  • DNA, Bacterial / genetics
  • DNA-Directed RNA Polymerases / chemistry
  • DNA-Directed RNA Polymerases / metabolism*
  • Dimerization
  • Electrochemistry
  • Enzyme Activation / genetics
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics*
  • Mutagenesis / physiology
  • Promoter Regions, Genetic / genetics*
  • Protein Binding / physiology
  • Protein Conformation
  • Protein Structure, Tertiary
  • Transcription, Genetic / physiology


  • Amino Acids
  • Cyclic AMP Receptor Protein
  • DNA, Bacterial
  • DNA-Directed RNA Polymerases