Kes1p shares homology with human oxysterol binding protein and participates in a novel regulatory pathway for yeast Golgi-derived transport vesicle biogenesis

EMBO J. 1996 Dec 2;15(23):6447-59.


The yeast phosphatidylinositol transfer protein (Sec14p) is required for biogenesis of Golgi-derived transport vesicles and cell viability, and this essential Sec14p requirement is abrogated by inactivation of the CDP-choline pathway for phosphatidylcholine biosynthesis. These findings indicate that Sec14p functions to alleviate a CDP-choline pathway-mediated toxicity to yeast Golgi secretory function. We now report that this toxicity is manifested through the action of yeast Kes1p, a polypeptide that shares homology with the ligand-binding domain of human oxysterol binding protein (OSBP). Identification of Kes1p as a negative effector for Golgi function provides the first direct insight into the biological role of any member of the OSBP family, and describes a novel pathway for the regulation of Golgi-derived transport vesicle biogenesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites
  • Carrier Proteins / metabolism
  • Cytidine Diphosphate Choline / metabolism
  • DNA Primers
  • Fungal Proteins / chemistry
  • Fungal Proteins / metabolism*
  • Golgi Apparatus / physiology*
  • Golgi Apparatus / ultrastructure
  • Humans
  • Membrane Proteins*
  • Phosphatidylinositols / metabolism
  • Phospholipid Transfer Proteins
  • Polymerase Chain Reaction
  • Receptors, Steroid / chemistry*
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae / ultrastructure*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid


  • Carrier Proteins
  • DNA Primers
  • Fungal Proteins
  • KES1 protein, S cerevisiae
  • Membrane Proteins
  • Phosphatidylinositols
  • Phospholipid Transfer Proteins
  • Receptors, Steroid
  • Recombinant Proteins
  • SEC24 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • oxysterol binding protein
  • Cytidine Diphosphate Choline