Antagonistic actions of activin A and BMP-2/4 control dorsal lip-specific activation of the early response gene XFD-1' in Xenopus laevis embryos

EMBO J. 1996 Dec 2;15(23):6739-49.

Abstract

Transcription of the early response gene XFD-1' (XFKH1) in the dorsal lip (Spemann organizer) of Xenopus embryos is activated by dorsal mesoderm inducing factors. Promoter studies revealed the presence of an activin A response element (ARE) which is both necessary and sufficient for transcriptional activation of reporter genes in animal cap explants incubated with activin A. Surprisingly, this ARE is also active within vegetal explants in the absence of exogenously added inducers, but an additional inhibitory response element prevents transcription of the XFD-1' gene in the ventral/vegetal region of the embryo in vivo. This element is located upstream of the ARE, it responds to bone morphogenic proteins 2 and 4 (BMP-2/4) triggered signals and it overrides the activating properties of the ARE. Expression patterns of BMP-2 and BMP-4 in the late blastula stage embryo and, especially, their absence from the dorsal blastopore lip may thus control the spatial transcription of the XFD-1' gene. Accordingly, the temporal activation and the spatial restriction of XFD-1' gene activity to the Spemann organizer is regulated by antagonistic actions of two distinct members of the TGF-beta family (activin and BMP) which act on different promoter elements.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Activins
  • Animals
  • Base Sequence
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Protein 4
  • Bone Morphogenetic Proteins / biosynthesis*
  • DNA-Binding Proteins / biosynthesis*
  • DNA-Binding Proteins / genetics
  • Embryo, Nonmammalian / physiology*
  • Forkhead Transcription Factors
  • Gene Expression Regulation, Developmental*
  • Inhibins / biosynthesis*
  • Lip
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins / biosynthesis
  • Regulatory Sequences, Nucleic Acid
  • Sequence Deletion
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic
  • Transforming Growth Factor beta*
  • Xenopus Proteins*
  • Xenopus laevis

Substances

  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Protein 4
  • Bone Morphogenetic Proteins
  • DNA-Binding Proteins
  • Forkhead Transcription Factors
  • FoxA4 protein, Xenopus
  • Recombinant Fusion Proteins
  • Transforming Growth Factor beta
  • Xenopus Proteins
  • bmp4 protein, Xenopus
  • foxd4l1.1 protein, Xenopus
  • Activins
  • Inhibins

Associated data

  • GENBANK/U65750
  • GENBANK/U65751