Amino acids of the alpha1B-adrenergic receptor involved in agonist binding: differences in docking catecholamines to receptor subtypes

FEBS Lett. 1996 Dec 9;399(1-2):9-13. doi: 10.1016/s0014-5793(96)01286-0.


Site-directed mutagenesis and molecular dynamics analysis of the 3-D model of the alpha1B-adrenergic receptor (AR) were combined to identify the molecular determinants of the receptor involved in catecholamine binding. Our results indicate that the three conserved serines in the fifth transmembrane domain (TMD) of the alpha1B-AR play a distinct role in catecholamine binding versus receptor activation. In addition to the amino acids D125 in TMDIII and S207 in TMDV directly involved in ligand binding, our findings identify a large number of polar residues playing an important role in the activation process of the alpha1B-AR thus providing new insights into the structure/function relationship of G protein-coupled receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenergic alpha-1 Receptor Agonists*
  • Adrenergic alpha-Agonists / pharmacology*
  • Amino Acids / chemistry*
  • Amino Acids / genetics
  • Animals
  • COS Cells
  • Cricetinae
  • Epinephrine / metabolism*
  • Mutagenesis, Site-Directed
  • Protein Binding
  • Receptors, Adrenergic, alpha-1 / chemistry
  • Receptors, Adrenergic, alpha-1 / genetics


  • Adrenergic alpha-1 Receptor Agonists
  • Adrenergic alpha-Agonists
  • Amino Acids
  • Receptors, Adrenergic, alpha-1
  • Epinephrine