Subregion- and cell type-restricted gene knockout in mouse brain

Cell. 1996 Dec 27;87(7):1317-26. doi: 10.1016/s0092-8674(00)81826-7.


Using the phage P1-derived Cre/loxP recombination system, we have developed a method to create mice in which the deletion (knockout) of virtually any gene of interest is restricted to a subregion or a specific cell type in the brain such as the pyramidal cells of the hippocampal CA1 region. The Cre/loxP recombination-based gene deletion appears to require a certain level of Cre protein expression. The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics
  • Gene Expression
  • Gene Expression Regulation
  • Genetic Engineering / methods*
  • Gestational Age
  • Hippocampus / physiology*
  • Integrases / genetics*
  • Mice
  • Mice, Knockout / genetics*
  • Mice, Transgenic
  • Promoter Regions, Genetic
  • RNA, Messenger / genetics
  • Recombination, Genetic
  • Sequence Deletion
  • Viral Proteins*


  • RNA, Messenger
  • Viral Proteins
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Cre recombinase
  • Integrases