Coordinated Activation of as-1-type Elements and a Tobacco Glutathione S-transferase Gene by Auxins, Salicylic Acid, Methyl-Jasmonate and Hydrogen Peroxide

Plant Mol Biol. 1996 Nov;32(3):415-26. doi: 10.1007/BF00019093.

Abstract

The molecular mechanism of signal transduction pathways which mediate the action of phytohormones are poorly understood. Recently, we and others have shown that the as -1 type cis-acting elements can respond to auxin and salicylic acid, two well-characterized signaling molecules in plants. In the present work, we have examined a comprehensive set of physiological and abiotic agents and found that auxin, salicylic acid and methyl-jasmonate are three effective inducers of the as-1-type elements in transgenic tobacco. Using a cell suspension culture containing a synthetic promoter-GUS fusion, we demonstrated rapid and sensitive induction of the as-1-type element by these phytohormones. Furthermore, a tobacco glutathione S-transferase gene, GNT35, that contains an as-1-type binding site in its promoter is also inducible by auxin, salicylic acid and methyl-jasmonate with similar kinetics. As Ulmasov et al. have recently reported, we found that the as-1-type elements can also respond to weak/inactive analogues of auxin and salicylic acid. In addition, we show that hydrogen peroxide can also effectively activate the expression of GNT35 as well as the as-1-type element in a cell suspension culture, but not with whole seedlings. These results are discussed with respect to the possible mechanism(s) through which a single cis element may respond to a diverse array of molecules.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetates / pharmacology
  • Binding Sites
  • Cyclopentanes / pharmacology
  • Gene Expression Regulation, Plant / drug effects*
  • Genes, Reporter / genetics
  • Glucuronidase / genetics
  • Glutathione Transferase / genetics
  • Hydrogen Peroxide / pharmacology
  • Indoleacetic Acids / pharmacology
  • Oxylipins
  • Plant Growth Regulators / pharmacology*
  • Plants, Genetically Modified
  • Plants, Toxic*
  • Promoter Regions, Genetic / genetics
  • RNA, Messenger / analysis
  • RNA, Plant / analysis
  • Regulatory Sequences, Nucleic Acid / genetics*
  • Salicylates / pharmacology
  • Salicylic Acid
  • Signal Transduction / genetics*
  • Tobacco / genetics*

Substances

  • Acetates
  • Cyclopentanes
  • Indoleacetic Acids
  • Oxylipins
  • Plant Growth Regulators
  • RNA, Messenger
  • RNA, Plant
  • Salicylates
  • methyl jasmonate
  • Hydrogen Peroxide
  • Glutathione Transferase
  • Glucuronidase
  • Salicylic Acid