A molecular study of dormancy breaking and germination in seeds of Trollius ledebouri

Plant Mol Biol. 1996 Nov;32(3):559-64. doi: 10.1007/BF00019110.

Abstract

A cDNA library was generated from seeds of Trollius ledebouri cv. Golden Queen after GA3 treatment. Five clones encoded mRNAs which were down-regulated during dormancy breaking and the initial stages of germination. Two of these showed homology to storage proteins (pPCB3 and pPCB4) and one each to the late-embryogenesis-abundant (LEA) group 2 dehydrin proteins (pPCB2), a barely glucose dehydrogenase (pPCB6) and the glutathione S-transferase (GST) superfamily (pPCB7). Transcript levels declined over 8 days in GA3-treated seeds. In dormant imbibed seeds transcript levels were relatively unchanged over the same period except for the PCB3 transcript, the level of which increased.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • DNA, Complementary / genetics
  • DNA, Plant / genetics
  • Gene Expression Regulation, Plant / drug effects
  • Gene Expression Regulation, Plant / physiology*
  • Gene Library
  • Germination / genetics*
  • Gibberellins / pharmacology
  • Glucose 1-Dehydrogenase
  • Glucose Dehydrogenases / genetics
  • Glutathione Transferase / genetics
  • Molecular Sequence Data
  • Plant Proteins / genetics
  • RNA, Messenger / analysis
  • RNA, Plant / analysis
  • Seeds / genetics*
  • Seeds / physiology
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • DNA, Plant
  • Gibberellins
  • Plant Proteins
  • RNA, Messenger
  • RNA, Plant
  • late embryogenesis abundant protein, plant
  • Glucose Dehydrogenases
  • Glucose 1-Dehydrogenase
  • Glutathione Transferase

Associated data

  • GENBANK/Z69994
  • GENBANK/Z69995
  • GENBANK/Z69996