Abstract
A cDNA library was generated from seeds of Trollius ledebouri cv. Golden Queen after GA3 treatment. Five clones encoded mRNAs which were down-regulated during dormancy breaking and the initial stages of germination. Two of these showed homology to storage proteins (pPCB3 and pPCB4) and one each to the late-embryogenesis-abundant (LEA) group 2 dehydrin proteins (pPCB2), a barely glucose dehydrogenase (pPCB6) and the glutathione S-transferase (GST) superfamily (pPCB7). Transcript levels declined over 8 days in GA3-treated seeds. In dormant imbibed seeds transcript levels were relatively unchanged over the same period except for the PCB3 transcript, the level of which increased.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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DNA, Complementary / genetics
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DNA, Plant / genetics
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Gene Expression Regulation, Plant / drug effects
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Gene Expression Regulation, Plant / physiology*
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Gene Library
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Germination / genetics*
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Gibberellins / pharmacology
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Glucose 1-Dehydrogenase
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Glucose Dehydrogenases / genetics
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Glutathione Transferase / genetics
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Molecular Sequence Data
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Plant Proteins / genetics
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RNA, Messenger / analysis
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RNA, Plant / analysis
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Seeds / genetics*
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Seeds / physiology
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Sequence Homology, Amino Acid
Substances
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DNA, Complementary
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DNA, Plant
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Gibberellins
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Plant Proteins
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RNA, Messenger
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RNA, Plant
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late embryogenesis abundant protein, plant
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Glucose Dehydrogenases
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Glucose 1-Dehydrogenase
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Glutathione Transferase
Associated data
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GENBANK/Z69994
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GENBANK/Z69995
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GENBANK/Z69996