Isolation and characterization of cDNA clones corresponding to the genes expressed preferentially in floral organs of Arabidopsis thaliana

Plant Mol Biol. 1996 Nov;32(4):759-65. doi: 10.1007/BF00020217.

Abstract

Seventeen cDNA clones of genes corresponding to mRNAs expressed preferentially in floral organs of Arabidopsis thaliana were obtained by differential screening of a flower bud cDNA library, and classified into five groups (1A, 17A, 1B, 4B and 5B) by cross-hybridization and restriction analysis. Sequence analysis revealed that the 1A-1 and 17A-1 clones encode vegetative storage proteins (VSPs). The VSP mRNAs were detected in a small amount in leaves and increased to a limited level by wounding. Both 1B-1 and 5B-1 clones were homologous to transmembrane protein cDNAs. The protein encoded by 4B-1 clone contained a proline-rich region, but no homologous proteins were found in databases.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Cloning, Molecular
  • DNA, Complementary / genetics*
  • DNA, Plant / genetics*
  • Gene Expression Regulation, Plant / physiology*
  • Molecular Sequence Data
  • Plant Leaves / chemistry
  • RNA, Messenger / analysis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid

Substances

  • DNA, Complementary
  • DNA, Plant
  • RNA, Messenger

Associated data

  • GENBANK/D85190
  • GENBANK/D85191
  • GENBANK/D85192
  • GENBANK/D85193
  • GENBANK/D85194
  • GENBANK/X79490
  • SWISSPROT/P27061