Chemokine-dependent upregulation of CD11b on specific leukocyte subpopulations in human whole blood: effect of anticoagulant on rantes and MIP-1 beta stimulation

Cytokine. 1996 Oct;8(10):762-6. doi: 10.1006/cyto.1996.0101.


The effect of anticoagulant (heparin vs EDTA) on chemokine induced CD11b upregulation on neutrophils, eosinophils, and monocytes in human whole blood was determined. For most of the chemokines (IL-8, GRO-alpha, MCP-1, MIP-1 alpha) the difference in the response of leukocytes in EDTA anticoagulated blood vs those in heparinized blood was the degree of their maximal response, with a slightly higher maximal increase in CD11b expression usually seen in cells from EDTA anticoagulated blood. Two chemokines were exceptions to this: RANTES and MIP-1 beta. RANTES is considered to be a stimulator of monocytes and eosinophils and not of neutrophils. As expected, neutrophils in heparinized whole blood did not respond to RANTES; however, neutrophils in EDTA anticoagulated blood had a significant increase in CD11b when exposed to high concentrations (1 microM) of RANTES. RANTES-induced CD11b expression on monocytes and eosinophils in these samples were the same in either heparin or EDTA. In EDTA anticoagulated blood, MIP-1 beta did not elicit a response in either monocytes, eosinophils or neutrophils; however, in heparinized blood, all three cell types increased CD11b expression upon exposure to 1 microM MIP-1 beta.

MeSH terms

  • Chemokine CCL2 / pharmacology
  • Chemokine CCL4
  • Chemokine CCL5 / metabolism*
  • Chemokine CCL7
  • Chemokine CCL8
  • Chemokine CXCL1
  • Chemokines / pharmacology*
  • Chemokines, CXC*
  • Chemotactic Factors / pharmacology
  • Cytokines*
  • Eosinophils / drug effects
  • Flow Cytometry
  • Growth Substances / pharmacology
  • Heparin / pharmacology*
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Interleukin-8 / pharmacology
  • Leukocytes / cytology*
  • Leukocytes / drug effects
  • Macrophage Inflammatory Proteins / pharmacology*
  • Macrophage-1 Antigen / pharmacology*
  • Monocyte Chemoattractant Proteins / pharmacology
  • Monocytes / drug effects
  • Neutrophils / drug effects
  • Up-Regulation / drug effects*


  • CCL7 protein, human
  • CCL8 protein, human
  • CXCL1 protein, human
  • Chemokine CCL2
  • Chemokine CCL4
  • Chemokine CCL5
  • Chemokine CCL7
  • Chemokine CCL8
  • Chemokine CXCL1
  • Chemokines
  • Chemokines, CXC
  • Chemotactic Factors
  • Cytokines
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-8
  • Macrophage Inflammatory Proteins
  • Macrophage-1 Antigen
  • Monocyte Chemoattractant Proteins
  • Heparin