Cloning and functional characterization of a rat urea transporter: expression in the brain

Biochim Biophys Acta. 1996 Dec 11;1309(3):197-9. doi: 10.1016/s0167-4781(96)00172-8.

Abstract

A cDNA coding for a rat urea transporter is described. The 1242 bp open reading frame codes for a 414 amino acids protein with 77.0% and 60.7% identity with the human UT11 and the rat UT2, respectively. When expressed in Xenopus oocytes, the protein induces a 10-fold rise in urea permeability, inhibited by mercurial reagents and phloretin. The rUT11 mRNA is massively expressed in the brain.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Brain / metabolism
  • Carrier Proteins / chemistry*
  • Cloning, Molecular
  • Gene Expression Regulation / genetics
  • Gene Library
  • Glycosylation
  • Molecular Sequence Data
  • Oocytes / metabolism
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • Thiourea / metabolism
  • Urea / metabolism*
  • Xenopus laevis

Substances

  • Carrier Proteins
  • RNA, Messenger
  • Urea
  • Thiourea

Associated data

  • GENBANK/X98399