Immune response to cyclin B1 in hepatocellular carcinoma

Hepatology. 1997 Jan;25(1):75-80. doi: 10.1002/hep.510250114.


Proteins expressed by plasmids encoding human cyclins and cyclin-dependent kinase 2 (CDK2) were used as antigens in immunoblotting. Fifteen of 100 patients with hepatocellular carcinoma (HCC) were found to have autoantibodies reactive with cyclin B1 and with a 40-kd degradation product of cyclin B1-glutathione-S-transferase (GST) fusion protein. Only one serum was found to react with cyclin A and another single serum with CDK2 but no antibodies were detected to cyclin D1 and E. The basis for autoimmune responses to cyclin B1 in HCC are unknown at the present time but the possibilities might include aberrations in cyclin B1 regulation leading to altered product or its expression which resulted in stimulation of immune reactions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Autoantibodies / blood*
  • Carcinoma, Hepatocellular / immunology*
  • Cyclin B*
  • Cyclin B1
  • Cyclins / immunology*
  • Genes, p53
  • Humans
  • Immunoblotting
  • Liver Neoplasms / immunology*
  • Molecular Weight
  • Mutation
  • Rabbits


  • Autoantibodies
  • CCNB1 protein, human
  • Cyclin B
  • Cyclin B1
  • Cyclins