Testis-specific expression of a novel human H3 histone gene

Exp Cell Res. 1996 Dec 15;229(2):301-6. doi: 10.1006/excr.1996.0375.


We have investigated the expression of a recently described, solitary human H3 histone gene. Using RNase protection assays, the corresponding mRNA could only be detected in RNA preparations from human testis, whereas several human cell lines and somatic tissues did not exhibit expression of this gene. In situ hybridization of sections from human testis revealed expression to be confined to primary spermatocytes. In addition to H1t, this novel H3 gene, which is located on chromosome 1, is the second tissue-specific human histone gene that has been found to be expressed solely in the testis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Base Sequence
  • Cell Line
  • DNA / genetics
  • Gene Expression
  • Histones / genetics*
  • Humans
  • In Situ Hybridization
  • Male
  • Organ Specificity
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Restriction Mapping
  • Testis / metabolism*


  • Histones
  • RNA, Messenger
  • DNA