IS431mec-mediated integration of a bleomycin-resistance gene into the chromosome of a methicillin-resistant Staphylococcus aureus strain isolated in Japan

Appl Microbiol Biotechnol. 1996 Aug;46(1):61-6. doi: 10.1007/s002530050783.


A methicillin-resistant Staphylococcus aureus (MRSA) strain B-26, isolated clinically in Hiroshima University Hospital, is resistant to bleomycin together with kanamycin. In the present study, we analysed the nucleotide sequence of the 5.1-kb HindIII fragment containing the bleomycin- and kanamycin-resistance genes, which were previously cloned [Bhuiyan et al. (1995) Appl Microbiol Biotechnol 43: 65-69] from the chromosomal DNA of MRSA B-26. The present study found that the DNA sequence contains the duplicated target sequence (GATTAGAT) consisting of 8 bp for transposase and the entire nucleotide sequence of the plasmid pUB110, together with the sequence of inverted repeats (16 bp), designated IR-r and IR-1 in IS431 mec. The 8-bp duplication sequence, produced by the transposable element, was first found by us. We proposed that bleomycin resistance in MRSA B-26 is attributed to the IS431 mec-mediated integration of pUB110 into the chromosome.

MeSH terms

  • Bacterial Proteins
  • Base Sequence / genetics
  • Bleomycin / pharmacology*
  • Chromosomes, Bacterial / genetics
  • DNA Transposable Elements / genetics*
  • Drug Resistance, Microbial / genetics
  • Drug Resistance, Multiple / genetics*
  • Genes, Bacterial / genetics
  • Japan
  • Kanamycin Resistance / genetics
  • Methicillin / pharmacology
  • Methicillin Resistance / genetics*
  • Molecular Sequence Data
  • Plasmids / genetics
  • Restriction Mapping
  • Sequence Analysis, DNA
  • Staphylococcus aureus / drug effects*
  • Staphylococcus aureus / genetics
  • Transformation, Bacterial


  • Bacterial Proteins
  • DNA Transposable Elements
  • Bleomycin
  • Methicillin