Characterization of Cryptococcus neoformans by random DNA amplification

Lett Appl Microbiol. 1996 Nov;23(5):312-6. doi: 10.1111/j.1472-765x.1996.tb00197.x.


Random amplified polymorphic DNA (RAPD) was optimized and used to distinguish between the varieties and serotypes of Cryptococcus neoformans. The RAPD technique distinguished between serotypes A, D or AD within C. neoformans var. neoformans, and revealed further differentiation within each serotype. Four RAPD profiles were clearly recognizable within C. neoformans var. gattii, although its two serotypes, B and C, were only differentiated with one primer combination out of seven.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cryptococcus neoformans / classification*
  • Cryptococcus neoformans / genetics
  • DNA, Fungal / analysis*
  • Random Amplified Polymorphic DNA Technique*


  • DNA, Fungal