Characterization of Cryptococcus neoformans by random DNA amplification

P Ruma; S C Chen; T C Sorrell; A G Brownlee

doi:10.1111/j.1472-765x.1996.tb00197.x

Characterization of Cryptococcus neoformans by random DNA amplification

Lett Appl Microbiol. 1996 Nov;23(5):312-6. doi: 10.1111/j.1472-765x.1996.tb00197.x.

Authors

P Ruma¹, S C Chen, T C Sorrell, A G Brownlee

Affiliation

¹ Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, NSW, Australia.

PMID: 8987712
DOI: 10.1111/j.1472-765x.1996.tb00197.x

Abstract

Random amplified polymorphic DNA (RAPD) was optimized and used to distinguish between the varieties and serotypes of Cryptococcus neoformans. The RAPD technique distinguished between serotypes A, D or AD within C. neoformans var. neoformans, and revealed further differentiation within each serotype. Four RAPD profiles were clearly recognizable within C. neoformans var. gattii, although its two serotypes, B and C, were only differentiated with one primer combination out of seven.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cryptococcus neoformans / classification*
Cryptococcus neoformans / genetics
DNA, Fungal / analysis*
Random Amplified Polymorphic DNA Technique*

Substances

DNA, Fungal