Projection structure of a gap junction membrane channel at 7 A resolution

Nat Struct Biol. 1997 Jan;4(1):39-43. doi: 10.1038/nsb0197-39.

Abstract

Electron cryo-microscopy and image analysis of frozen-hydrated, two-dimensional crystals of gap junction membrane channels formed by recombinant alpha 1 connexin (Cx43) reveal a ring of transmembrane alpha-helices that lines the aqueous pore and a second ring of alpha-helices in close contact with the membrane lipids.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Connexin 43 / chemistry*
  • Connexin 43 / metabolism
  • Cricetinae
  • Crystallography, X-Ray
  • Membrane Lipids / metabolism
  • Microscopy, Electron / methods
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism

Substances

  • Connexin 43
  • Membrane Lipids
  • Recombinant Proteins