Tn5431 arose by transposition of Tn3 into Tn1721

Can J Microbiol. 1996 Dec;42(12):1274-6. doi: 10.1139/m96-166.


To further characterize Tn5431, which is composed of Tn3 and Tn1721, DNA sequencing was carried out. It was demonstrated that Tn5431 arose by transposition of Tn3 into Tn1721. Multiple mutations in the Tn3 tnpA gene have occurred, rendering the Tn3 portion of Tn5431 transposition defective and ensuring the simultaneous transposition of the antibiotic resistance determinants on Tn3 and Tn1721.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents / pharmacology
  • Base Sequence
  • Cloning, Molecular
  • Conjugation, Genetic
  • DNA Transposable Elements / genetics*
  • DNA-Binding Proteins / genetics
  • Drug Resistance, Microbial
  • Escherichia coli / genetics*
  • Molecular Sequence Data
  • Plant Proteins*
  • Plasmids / genetics*
  • Recombination, Genetic*
  • Repressor Proteins / genetics
  • Restriction Mapping
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid


  • Anti-Bacterial Agents
  • DNA Transposable Elements
  • DNA-Binding Proteins
  • Plant Proteins
  • Repressor Proteins

Associated data

  • GENBANK/X87813
  • GENBANK/X87814