Maintenance of broad-host-range incompatibility group P and group Q plasmids and transposition of Tn5 in Bartonella henselae following conjugal plasmid transfer from Escherichia coli

C Dehio; M Meyer

doi:10.1128/jb.179.2.538-540.1997

Maintenance of broad-host-range incompatibility group P and group Q plasmids and transposition of Tn5 in Bartonella henselae following conjugal plasmid transfer from Escherichia coli

J Bacteriol. 1997 Jan;179(2):538-40. doi: 10.1128/jb.179.2.538-540.1997.

Authors

C Dehio¹, M Meyer

Affiliation

¹ Unité de Pathogenie Microbienne Moléculaire, Institut Pasteur, Paris,France. dehio@mpib-tuebingen.mpg.de

Abstract

The first demonstration of conjugal plasmid transfer from Escherichia coli to Bartonella henselae is reported. Transconjugants bearing plasmids of incompatibility groups P (IncP) and Q (IncQ), expressing various resistance markers, were generated. Tn5 transposons delivered on suicide plasmids by conjugation showed transpositional insertion into random chromosomal sites.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bartonella henselae / genetics
Conjugation, Genetic*
DNA Nucleotidyltransferases*
DNA, Bacterial*
Escherichia coli / genetics*
Plasmids
Transposases

Substances

DNA, Bacterial
DNA Nucleotidyltransferases
Transposases