[CpG motif in DNA from immune complexes of SLE patients augments expression of intercellular adhesion molecule-1 on endothelial cells]

Rinsho Byori. 1996 Dec;44(12):1125-31.
[Article in Japanese]


The sequence of 9 DNA clones obtained from DNA-anti-DNA antibody immune complexes (IC) in 11 SLE patients was analyzed and the possible pathogenic role of the circulating DNA in SLE patients was discussed. Nucleic acid length of 9 cloned DNAs ranged from 87 to 312 base pairs(bp), with a mean length of 177 +/- 62bp, which were rich in guanine (G) + cytosine(C), CpG dinucleotide and palindromic sequences. Oligonucleotide TTTTCAATTCGAAGATGATT which contain the CpG motif in hexamer palindromic sequence segments in cloned DNA augmented the expression of ICAM-1 on the endothelial cells detected by FACS analysis and also augmented the gene expression of several cytokines such as interleukin-2, interleukin-6, interleukin-8 and tumor necrosis factor alpha. These data suggest that DNA in IC of SLE patients will augment expression of ICAM-1 on endothelial cells, resulting in exacerbation of vasculitis.

Publication types

  • English Abstract
  • Review

MeSH terms

  • Antigen-Antibody Complex / genetics*
  • Base Sequence
  • DNA / genetics
  • DNA / physiology*
  • Endothelium, Vascular / metabolism*
  • Humans
  • Intercellular Adhesion Molecule-1 / metabolism*
  • Lupus Erythematosus, Systemic / etiology*
  • Molecular Sequence Data
  • Oligonucleotides
  • Vasculitis / etiology


  • Antigen-Antibody Complex
  • Oligonucleotides
  • Intercellular Adhesion Molecule-1
  • DNA