Cloning and Sequencing of the Gene Encoding the Cu,Zn-superoxide Dismutase of Haemophilus Ducreyi

Gene. 1996 Dec 12;183(1-2):35-40. doi: 10.1016/s0378-1119(96)00417-9.

Abstract

The sodC gene of Haemophilus ducreyi was cloned and sequenced. The deduced amino acid sequence of this protein exhibited 71.6% identity and 81.8% similarity to the H. influenzae and H. parainfluenzae copper (Cu), zinc (Zn)-superoxide dismutase (SOD) enzymes. This gene was localized to a 2.2-kb H. ducreyi chromosomal DNA insert in plasmid pHdSOD. SOD activity was expressed in cell-free extracts of Escherichia coli containing the recombinant plasmid pHdSOD and was localized to the periplasmic space. The Cu,Zn-SOD produced by the H. ducreyi sodC gene did not complement the aerobic growth defect of an E. coli SOD-deficient mutant.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Escherichia coli / growth & development
  • Genes, Bacterial / genetics*
  • Genetic Complementation Test
  • Haemophilus ducreyi / enzymology
  • Haemophilus ducreyi / genetics*
  • Molecular Sequence Data
  • Recombinant Fusion Proteins
  • Restriction Mapping
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Superoxide Dismutase / genetics*
  • Superoxide Dismutase / metabolism

Substances

  • Recombinant Fusion Proteins
  • Superoxide Dismutase

Associated data

  • GENBANK/U47664