Identification of a new prp locus required for propionate catabolism in Salmonella typhimurium LT2

FEMS Microbiol Lett. 1996 Apr 1;137(2-3):233-9. doi: 10.1111/j.1574-6968.1996.tb08111.x.


A new propionate (prp) locus of S. typhimurium was defined by mutation, was located to minute 8 of the chromosome, and was shown to be transcribed in the clockwise direction. A plasmid carrying the wild-type prp+ locus was isolated by complementation and its initial physical characterization is presented. Transcriptional regulation of prp was studied using MudI1734(lacZ+) operon fusions. Propionate stimulated prp transcription in a merodiploid strain containing prp+ and a prp::MudI1734 fusion, but failed to stimulate transcription of the same fusion in a haploid genetic background. prp transcription was reduced by a factor of 2 in strains deficient in the synthesis of the global regulatory protein FruR; fruR mutants failed to grow on propionate. Propionate blocked growth of prp mutants on medium containing succinate as carbon/energy source.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosome Mapping
  • Cloning, Molecular
  • Genes, Bacterial*
  • Lac Operon
  • Mutation
  • Phenotype
  • Propionates / metabolism*
  • Salmonella typhimurium / genetics*
  • Salmonella typhimurium / metabolism*
  • Succinates / metabolism
  • Succinic Acid
  • Transcription, Genetic


  • Propionates
  • Succinates
  • Succinic Acid