neurogenins, a novel family of atonal-related bHLH transcription factors, are putative mammalian neuronal determination genes that reveal progenitor cell heterogeneity in the developing CNS and PNS

Mol Cell Neurosci. 1996;8(4):221-41. doi: 10.1006/mcne.1996.0060.


We recently identified neurogenin (ngn), a neuroD-related bHLH gene, whose Xenopus homolog functions as a neuronal determination factor and upstream activator of XneuroD (Ma et al. Cell 87: 43-52, 1996). Here we identify two additional ngn's, ngn2 and ngn3, which together define a novel subfamily of atonal-related mouse genes. Comparative analysis of ngn expression indicates that these three genes define distinct progenitor populations in the developing CNS and PNS, exhibiting nonoverlapping expression in some areas and partial overlap in others. The expression of the ngn's spatially overlaps and often temporally precedes that of neuroD, suggesting that (as in Xenopus) the ngn's and neuroD function in a cascade. Thus, as in myogenesis, different bHLH determination factors may activate a common bHLH differentiation factor in different sublineages. The ngn's therefore represent both a family of putative mammalian neuronal determination genes and useful markers of the origins of neuronal diversity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • Central Nervous System / embryology*
  • Embryo, Mammalian / metabolism
  • Embryonic and Fetal Development
  • Gene Expression
  • Genetic Variation*
  • Islets of Langerhans / embryology
  • Mice / embryology
  • Mice / genetics
  • Molecular Sequence Data
  • Multigene Family*
  • Nerve Tissue Proteins*
  • Neurons / physiology*
  • Neuropeptides / genetics*
  • Neuropeptides / metabolism
  • Peripheral Nerves / embryology*
  • Stem Cells / physiology*
  • Transcription Factors / genetics


  • Basic Helix-Loop-Helix Transcription Factors
  • Nerve Tissue Proteins
  • Neurog3 protein, mouse
  • Neuropeptides
  • Transcription Factors

Associated data

  • GENBANK/U76207
  • GENBANK/U76208
  • GENBANK/U76209
  • GENBANK/U76210