Consumption of Eggs With Meals Increases the Susceptibility of Human Plasma and Low-Density Lipoprotein to Lipid Peroxidation

Ann Nutr Metab. 1996;40(5):243-51. doi: 10.1159/000177964.

Abstract

Consumption of eggs for a long period was shown to result in hypercholesterolemia and is generally restricted for this reason. In the present study we analyzed the effect of eggs consumption for 3 weeks on lipoprotein atherogenicity. Consumption of 2 eggs per day with the meals, for 3 weeks resulted in a minor elevation in plasma glucose and urea concentrations. Plasma cholesterol concentration increased by 11% (p < 0.05) as a result of increased plasma low-density lipoprotein (LDL) cholesterol levels. Plasma triglycerides decreased by 13% (p < 0.01), but there were no significant alterations in plasma apolipoproteins A-I or B-100 concentrations. Plasma high-density lipoprotein (HDL) cholesterol decreased by 11% (p < 0.05). There was a 13% reduction, though not significant, in the cholesterol efflux from J-774 A.1 macrophages by HDL that was derived after eggs consumption in comparison to HDL that was obtained at baseline. The susceptibility of plasma [using 100 mM of 2,2' azobis 2-amidinopropane (AAPH)] as well as that of LDL (using 10 microM of copper ions) to lipid peroxidation was increased by 42% and 34%, respectively, as measured by the thiobarbituric acid reactive substance (TBARS) assay (p < 0.01). Kinetic analysis of LDL oxidation by copper ions revealed a 37% reduction in the lag time required for the initiation of LDL oxidation after 3 weeks of eggs consumption. The total plasma fatty acids concentration increased from 2.2 +/- 0.5 to 3.2 +/- 0.6 mg/ml. The plasma antioxidants, vitamin E and carotenoids were not significantly affected by eggs consumption. We conclude that eggs consumption, in addition to its hypercholesterolemic effect, increases plasma and LDL oxidizability, a phenomenon which was shown to enhance the progression of atherosclerosis. The atherogenic properties may contribute to the accelerated atherosclerosis prevalent in populations with high cholesterol intake.

MeSH terms

  • Adult
  • Antioxidants / metabolism
  • Blood Glucose / metabolism
  • Blood Urea Nitrogen
  • Cholesterol / blood
  • Cholesterol, Dietary / administration & dosage
  • Diet*
  • Eggs*
  • Female
  • Humans
  • Lipid Peroxidation*
  • Lipoproteins, HDL / blood
  • Lipoproteins, LDL / blood*
  • Male
  • Malondialdehyde / metabolism
  • Middle Aged
  • Triglycerides / blood

Substances

  • Antioxidants
  • Blood Glucose
  • Cholesterol, Dietary
  • Lipoproteins, HDL
  • Lipoproteins, LDL
  • Triglycerides
  • Malondialdehyde
  • Cholesterol