High doses of alcohol increase urinary testosterone-to-epitestosterone ratio in females

J Chromatogr B Biomed Appl. 1996 Dec 6;687(1):109-16. doi: 10.1016/s0378-4347(96)00204-6.


The effect of alcohol (1.2 and 2.0 g/kg) on the urinary testosterone-to-epitestosterone (T/E) ratio was studied by two experiments each conducted with four healthy females and males. The intake of 2.0 g/kg of ethanol within 5 h in the evening significantly increased plasma testosterone concentration and ratio of T/E in urine collected next morning in females. The results suggest that alcohol increases the T/E ratio more in females than in males. The effect of high doses of alcohol on urinary T/E ratio must be kept in mind when doping tests are performed during training periods.

MeSH terms

  • Adrenal Glands / drug effects
  • Adult
  • Alcohol Drinking
  • Doping in Sports
  • Epitestosterone / blood
  • Epitestosterone / urine*
  • Ethanol / pharmacology*
  • Female
  • Gas Chromatography-Mass Spectrometry
  • Gonadal Steroid Hormones / blood
  • Gonadal Steroid Hormones / urine
  • Humans
  • Liver / drug effects
  • Male
  • Sex Characteristics
  • Substance Abuse Detection
  • Testosterone / blood
  • Testosterone / urine*


  • Gonadal Steroid Hormones
  • Ethanol
  • Testosterone
  • Epitestosterone