The structure of the exopolysaccharide produced by the halophilic Archaeon Haloferax mediterranei strain R4 (ATCC 33500)

Carbohydr Res. 1996 Dec 13;295:147-56. doi: 10.1016/s0008-6215(96)90134-2.

Abstract

The halophilic Archaeon Haloferax mediterranei exudes into the growth medium a high molecular weight sulfated polysaccharide. The structure of the repeating unit of this polymer was determined by a combination of glycose, methylation, and sulfate analysis, periodate oxidation, and 1D and 2D NMR spectroscopic analysis of the native and periodate-oxidised/reduced polysaccharides. The location of the sulfate group was established from the 1H and 13C NMR data. The structure of the repeating unit of the polysaccharide may be written as [formula: see text]

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylglucosamine / analogs & derivatives
  • Acetylglucosamine / analysis
  • Archaea / chemistry*
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Glucose / analysis
  • Magnetic Resonance Spectroscopy
  • Mannose / analysis
  • Molecular Sequence Data
  • Oligosaccharides / chemistry*
  • Periodic Acid / metabolism
  • Polysaccharides, Bacterial / chemistry*
  • Repetitive Sequences, Nucleic Acid
  • Sequence Analysis
  • Spectrometry, Mass, Fast Atom Bombardment
  • Sulfuric Acid Esters / chemistry*

Substances

  • Oligosaccharides
  • Polysaccharides, Bacterial
  • Sulfuric Acid Esters
  • Periodic Acid
  • metaperiodate
  • Glucose
  • Mannose
  • Acetylglucosamine