Abstract
The halophilic Archaeon Haloferax mediterranei exudes into the growth medium a high molecular weight sulfated polysaccharide. The structure of the repeating unit of this polymer was determined by a combination of glycose, methylation, and sulfate analysis, periodate oxidation, and 1D and 2D NMR spectroscopic analysis of the native and periodate-oxidised/reduced polysaccharides. The location of the sulfate group was established from the 1H and 13C NMR data. The structure of the repeating unit of the polysaccharide may be written as [formula: see text]
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylglucosamine / analogs & derivatives
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Acetylglucosamine / analysis
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Archaea / chemistry*
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Carbohydrate Conformation
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Carbohydrate Sequence
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Glucose / analysis
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Magnetic Resonance Spectroscopy
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Mannose / analysis
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Molecular Sequence Data
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Oligosaccharides / chemistry*
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Periodic Acid / metabolism
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Polysaccharides, Bacterial / chemistry*
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Repetitive Sequences, Nucleic Acid
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Sequence Analysis
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Spectrometry, Mass, Fast Atom Bombardment
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Sulfuric Acid Esters / chemistry*
Substances
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Oligosaccharides
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Polysaccharides, Bacterial
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Sulfuric Acid Esters
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Periodic Acid
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metaperiodate
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Glucose
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Mannose
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Acetylglucosamine