Details of T-DNA structural organization from a transgenic Petunia population exhibiting co-suppression

Plant Mol Biol. 1996 Dec;32(6):1197-203. doi: 10.1007/BF00041406.

Abstract

Analysis of Agrobacterium-transferred DNA (T-DNA) revealed strong correlations between transgene structures and floral pigmentation patterns from chalcone synthase (chs) co-suppression among 47 Petunia transformants. Presented here are the full details of T-DNA structural organization in that population. Sixteen transformants (34%) carried one T-DNA copy while 31 (66%) carried 106 complete and partial T-DNA elements in 54 linkage groups. Thirty linkage groups contained multiple T-DNA copies; 15 of these contained only contiguously repeated copies, 8 contained only dispersed copies and 7 contained both. Right-border inverted repeats were three times more frequent than left-border inverted or direct repeats. Large fragments of binary-vector sequences were linked to the T-DNA in seven plants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Southern
  • DNA Restriction Enzymes
  • DNA, Bacterial / analysis
  • DNA, Bacterial / chemistry*
  • Genetic Linkage
  • Genetic Vectors
  • Phenotype
  • Plants, Genetically Modified / genetics*
  • Repetitive Sequences, Nucleic Acid
  • Suppression, Genetic*
  • Transformation, Genetic

Substances

  • DNA, Bacterial
  • T-DNA
  • DNA Restriction Enzymes