Isolation of a carbon source-regulated gene from Ustilago maydis

Mol Gen Genet. 1996 Dec 13;253(3):342-52. doi: 10.1007/pl00008601.


We have isolated a carbon source-regulated gene from the phytopathogenic fungus Ustilago maydis by use of a promoter-probe vector. This gene, called crg1, is strongly induced by L-arabinose and efficiently repressed by D-glucose and D-xylose. The predicted 36.5-kDa mature crg1 gene product lacks similarity to known proteins but is likely to be secreted. Sequences required for regulated expression of a reporter gene are contained within a 3.6-kb fragment upstream of the crg1 gene. The promoter of crg1 fulfils requirements for an efficient controllable gene expression system in U. maydis

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabinose / metabolism
  • Base Sequence
  • Carbon / metabolism*
  • Cloning, Molecular
  • DNA, Fungal
  • Escherichia coli
  • Fungal Proteins / genetics*
  • Gene Expression Regulation, Fungal*
  • Glucose / metabolism
  • Molecular Sequence Data
  • Plasmids
  • Promoter Regions, Genetic
  • Transcription, Genetic
  • Ustilago / genetics*
  • Xylose / metabolism


  • CRG1 protein, Ustilago maydis
  • DNA, Fungal
  • Fungal Proteins
  • Carbon
  • Xylose
  • Arabinose
  • Glucose

Associated data

  • GENBANK/X92509