Cloning and initial characterization of human and mouse Spot 14 genes

FEBS Lett. 1997 Jan 13;401(1):38-42. doi: 10.1016/s0014-5793(96)01433-0.


The intricate regulation of Spot 14 expression in rat lipogenic tissues has provided a useful tool in studying nutritional and hormonal factors involved in transcription. To gain insight into its function and its possible involvement in human lipid disorders, we cloned human and mouse Spot 14 genes that shared with the rat gene a strong homology concerning the deduced amino acid sequence (81 and 94%, respectively) as well as the promoter region. The mouse promoter was characterized by transfection studies, while quantitative RT-PCR and in situ hybridization experiments showed that Spot 14 is expressed in human liver and, at a high level, in multiple symmetric lipomatosis nodules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA
  • Humans
  • In Situ Hybridization
  • Mice
  • Molecular Sequence Data
  • Nuclear Proteins
  • Polymerase Chain Reaction
  • Proteins / genetics*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Transcription Factors


  • Nuclear Proteins
  • Proteins
  • THRSP protein, human
  • Thrsp protein, mouse
  • Thrsp protein, rat
  • Transcription Factors
  • DNA

Associated data

  • GENBANK/X95279
  • GENBANK/Y08409