Endothelin-1 induces GM-CSF, IL-6 and IL-8 but not G-CSF release from a human bronchial epithelial cell line (BEAS-2B)

Neuropeptides. 1996 Dec;30(6):551-6. doi: 10.1016/s0143-4179(96)90038-4.


Endothelin (ET) is a powerful vasoconstrictor and bronchoconstrictor peptide that may be involved in the pathogenesis of bronchial asthma. We have investigated the effect of ET on the secretion of IL-6, IL-8, GM-CSF and G-CSF in a bronchial epithelial cell line (BEAS-2B). Incubation of BEAS-2B cells with ET-1 (10(-13) to 10(-7) M) for 4 h caused dose-related increases in the release of IL-8 (68% increase above control, P < 0.001) and IL-6 (43% increase above control, P < 0.001), compared to untreated control cells. After 48 h incubation, ET-1 also increased the release of IL-8 by 35% (P < 0.001) and GM-CSF by 38% (P < 0.01). ET-1 had no significant effect on G-CSF release. ET-1 did not induce cell proliferation at 24 or 48 h. Since ET-immunoreactive materials are expressed in epithelial cells in asthma, it is possible that ET-1 of epithelial origin may act in a paracrine or autocrine fashion on airway epithelial ET receptors to stimulate IL-8, IL-N6 and GM-CSF release. Thus, ET-1 may play a role in the regulation of the cytokine responses involved in inflammation of the airway mucosa.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bronchi / cytology
  • Bronchi / drug effects
  • Bronchi / metabolism*
  • Cell Division / drug effects
  • Cell Line
  • Dose-Response Relationship, Drug
  • Endothelin-1 / pharmacology*
  • Endothelin-1 / physiology
  • Enzyme-Linked Immunosorbent Assay
  • Epithelial Cells
  • Epithelium / drug effects
  • Epithelium / metabolism
  • Granulocyte-Macrophage Colony-Stimulating Factor / drug effects
  • Granulocyte-Macrophage Colony-Stimulating Factor / immunology
  • Granulocyte-Macrophage Colony-Stimulating Factor / metabolism*
  • Humans
  • Interleukin-6 / immunology
  • Interleukin-6 / metabolism*
  • Interleukin-8 / immunology
  • Interleukin-8 / metabolism*
  • Time Factors


  • Endothelin-1
  • Interleukin-6
  • Interleukin-8
  • Granulocyte-Macrophage Colony-Stimulating Factor