Strand breaks in plasmid DNA following positional changes of Auger-electron-emitting radionuclides

Acta Oncol. 1996;35(7):797-801. doi: 10.3109/02841869609104029.

Abstract

The purpose of our studies is to elucidate the kinetics of DNA strand breaks caused by low-energy Auger electron emitters in close proximity to DNA. Previously we have studied the DNA break yields in plasmids after the decay of indium-111 bound to DNA or free in solution. In this work, we compare the DNA break yields in supercoiled DNA of iodine-125 decaying close to DNA following DNA intercalation, minor-groove binding, or surface binding, and at a distance from DNA. Supercoiled DNA, stored at 4 degrees C to accumulate radiation dose from the decay of 125I, was then resolved by gel electrophoresis into supercoiled, nicked circular, and linear forms, representing undamaged DNA, single-strand breaks, and double-strand breaks respectively. DNA-intercalated or groove-bound 125I is more effective than surface-bound radionuclide or 125I free in solution. The hydroxyl radical scavenger DMSO protects against damage by 125I free in solution but has minimal effect on damage by groove-bound 125I.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA Damage*
  • DNA, Superhelical / metabolism
  • DNA, Superhelical / radiation effects*
  • Indium Radioisotopes / metabolism
  • Iodine Radioisotopes* / metabolism
  • Linear Energy Transfer*
  • Plasmids / genetics
  • Plasmids / radiation effects*

Substances

  • DNA, Superhelical
  • Indium Radioisotopes
  • Iodine Radioisotopes