High-performance liquid chromatographic determination of baclofen in human plasma

J Chromatogr A. 1996 Apr 5;729(1-2):309-14. doi: 10.1016/0021-9673(95)00944-2.


A reversed-phase isocratic HPLC method is described for the determination of baclofen in human plasma. Solid-phase extraction using a SCX Bond Elut column is used followed by derivatization with o-phthalaldehyde-tert.-butanethiol and electrochemical detection. Both the within- and between-day R.S.D. and inaccuracy are less than 10% and 7%, respectively, even at the limit of quantification of the method, i.e., 10 ng/ml. The method was shown to give optimum performance in terms of sensitivity, precision and accuracy for the pharmacokinetic study of baclofen after a single oral administration to volunteers.

Publication types

  • Clinical Trial

MeSH terms

  • Baclofen / blood*
  • Baclofen / pharmacokinetics
  • Calibration
  • Chromatography, High Pressure Liquid
  • Electrochemistry
  • GABA Agonists / blood*
  • GABA Agonists / pharmacokinetics
  • Humans
  • Indicators and Reagents
  • Quality Control
  • Reference Standards
  • Reproducibility of Results


  • GABA Agonists
  • Indicators and Reagents
  • Baclofen