Abstract
We report on the characterization of the first loss-of-function mutation in a Drosophila CREB gene, dCREB-A. In the epidermis, dCREB-A is required for patterning cuticular structures on both dorsal and ventral surfaces since dCREB-A mutant larvae have only lateral structures around the entire circumference of each segment. Based on results from epistasis tests with known dorsal/ventral patterning genes, we propose that dCREB-A encodes a transcription factor that functions near the end of both the DPP- and SPI-signaling cascades to translate the corresponding extracellular signals into changes in gene expression. The lateralizing phenotype of dCREB-A mutants reveals a much broader function for CREB proteins than previously thought.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Chromosome Mapping
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Cyclic AMP Response Element-Binding Protein / biosynthesis
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Cyclic AMP Response Element-Binding Protein / genetics*
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Cyclic AMP Response Element-Binding Protein A
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DNA Primers
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / genetics*
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Drosophila Proteins*
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Drosophila melanogaster / embryology
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Drosophila melanogaster / genetics*
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Drosophila melanogaster / growth & development
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Embryo, Nonmammalian / physiology
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Embryonic Induction
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Enhancer Elements, Genetic
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Genes, Insect*
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Genetic Complementation Test
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Homozygote
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Larva
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Mutation
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Phenotype
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Polymerase Chain Reaction
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Salivary Glands / cytology
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Salivary Glands / physiology
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Trans-Activators / biosynthesis
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Trans-Activators / genetics*
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Transcription Factors*
Substances
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CrebA protein, Drosophila
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Cyclic AMP Response Element-Binding Protein
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Cyclic AMP Response Element-Binding Protein A
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DNA Primers
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DNA-Binding Proteins
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Drosophila Proteins
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Trans-Activators
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Transcription Factors