Purification of ADAM 10 from bovine spleen as a TNFalpha convertase

FEBS Lett. 1997 Jan 6;400(3):333-5. doi: 10.1016/s0014-5793(96)01410-x.

Abstract

We have purified a protease with characteristics of TNFalpha convertase from bovine spleen membranes. Peptide sequencing of the purified protein identified it as ADAM 10 (Genbank accession no. Z21961). This metalloprotease cleaves a recombinant proTNFalpha substrate to mature TNFalpha, and can cleave a synthetic peptide substrate to yield the mature TNFalpha amino terminus in vitro. The enzyme is sensitive to a hydroxamate inhibitor of MMPs, but insensitive to phosphoramidon. In addition, cloned ADAM 10 mediates proTNFalpha processing in a processing-incompetent cell line.

MeSH terms

  • ADAM Proteins
  • ADAM10 Protein
  • ADAM17 Protein
  • Amino Acid Sequence
  • Amyloid Precursor Protein Secretases
  • Animals
  • Cattle
  • Cell Line
  • Cloning, Molecular
  • Edetic Acid / pharmacology
  • Enzyme Inhibitors / pharmacology
  • Glycopeptides / pharmacology
  • Humans
  • Hydroxamic Acids / pharmacology
  • Membrane Proteins / genetics
  • Membrane Proteins / isolation & purification*
  • Membrane Proteins / metabolism
  • Metalloendopeptidases / genetics
  • Metalloendopeptidases / isolation & purification*
  • Metalloendopeptidases / metabolism
  • Molecular Sequence Data
  • Protein Precursors / metabolism
  • Recombinant Proteins / metabolism
  • Spleen / enzymology*
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Enzyme Inhibitors
  • Glycopeptides
  • Hydroxamic Acids
  • Membrane Proteins
  • Protein Precursors
  • Recombinant Proteins
  • SCH 43534
  • Tumor Necrosis Factor-alpha
  • tumor necrosis factor precursor
  • Edetic Acid
  • Amyloid Precursor Protein Secretases
  • ADAM Proteins
  • Metalloendopeptidases
  • ADAM 10 protein, Bos taurus
  • ADAM10 Protein
  • ADAM10 protein, human
  • ADAM17 Protein
  • phosphoramidon

Associated data

  • GENBANK/Z21961