Enhanced delivery of synthetic oligonucleotides to human leukaemic cells by liposomes and immunoliposomes

Leuk Res. Nov-Dec 1996;20(11-12):925-30. doi: 10.1016/s0145-2126(96)00062-8.

Abstract

The ability of pH-sensitive liposomes and immunoliposomes to deliver synthetic antisense oligonucleotides (oligos) into human myeloid and lymphoid leukaemia cells was examined. The cellular uptake of an 18mer anti-myb oligonucleotide encapsulated in liposomes was from three- to five-fold higher than that of 32P-oligos alone. In addition, anti-CD32 or anti-CD2 immunoliposomes improved the delivery of oligos to leukaemic cells carrying the appropriate receptor for the specific antibody-linked immunoliposome. The uptake of oligos was twice that of the liposome or non-specific immunoliposome encapsulated oligos. These findings support the use of liposomes or immunoliposomes to deliver antisense oligos into human leukaemic cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cholesterol
  • Drug Carriers
  • Glucosides
  • HL-60 Cells / metabolism
  • Humans
  • Hydrogen-Ion Concentration
  • Immunoconjugates / administration & dosage*
  • Leukemia / pathology*
  • Liposomes*
  • Neoplastic Stem Cells / metabolism*
  • Oleic Acid
  • Oligonucleotides, Antisense / administration & dosage*
  • Oligonucleotides, Antisense / chemical synthesis
  • Phosphatidylethanolamines
  • Tumor Cells, Cultured

Substances

  • Drug Carriers
  • Glucosides
  • Immunoconjugates
  • Liposomes
  • Oligonucleotides, Antisense
  • Phosphatidylethanolamines
  • octyl-beta-D-glucoside
  • Oleic Acid
  • 1,2-dielaidoylphosphatidylethanolamine
  • Cholesterol