Identification of a salicylic acid-responsive element in the promoter of the tobacco pathogenesis-related beta-1,3-glucanase gene, PR-2d

Plant J. 1996 Dec;10(6):1089-101. doi: 10.1046/j.1365-313x.1996.10061089.x.

Abstract

The tobacco pathogenesis-related PR-2d gene encodes an acidic beta-1,3-glucanase. Expression of the PR-2d: uidA(GUS) chimeric gene is induced in leaves undergoing the hypersensitive resistance response to tobacco mosaic virus and after treatment with salicylic acid (SA), a chemical believed to play an important role(s) in disease resistance. We have constructed transgenic tobacco plants which carry various segments of the PR-2d promoter fused to a heterologous core 35S promoter driving the uidA(GUS) reporter gene. Their analysis indicates that sequences from -364 to -288 upstream of the PR-2d transcription start site confer a high level of activation by SA (20-fold). Mutations within this sequence, located between -339 and -333, depressed SA activation. This region is also required for the SA-inducibility of a truncated PR-2d:GUS chimeric gene. Contained within this region is a 25 bp element located between -348 and -324 which was specifically recognized by nuclear factors from tobacco leaves. No conclusive differences were observed in the ability of proteins in nuclear extracts from water-treated versus SA-treated plants to bind to this cis element in vitro.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Binding Sites
  • DNA Methylation
  • DNA Mutational Analysis
  • Gene Expression Regulation, Plant*
  • Genes, Reporter
  • Glucan 1,3-beta-Glucosidase
  • Immunity, Innate
  • Nuclear Proteins / metabolism
  • Plant Diseases / genetics
  • Plant Growth Regulators / pharmacology*
  • Plant Proteins / genetics*
  • Plants, Genetically Modified
  • Plants, Toxic
  • Promoter Regions, Genetic*
  • Salicylates / pharmacology*
  • Salicylic Acid
  • Sequence Deletion
  • Signal Transduction
  • Tobacco / enzymology
  • Tobacco / genetics
  • beta-Glucosidase / genetics*

Substances

  • Nuclear Proteins
  • Plant Growth Regulators
  • Plant Proteins
  • Salicylates
  • pathogenesis-related proteins, plant
  • beta-Glucosidase
  • Glucan 1,3-beta-Glucosidase
  • Salicylic Acid