A transcriptional activation domain of ATMYB2, a drought-inducible Arabidopsis Myb-related protein

Plant J. 1996 Dec;10(6):1145-8. doi: 10.1046/j.1365-313x.1996.10061145.x.


Trans-activation activity of ATMYB2, a drought-inducible Myb-related protein in Arabidopsis thaliana, was analyzed using a transient assay of Arabidopsis leaf protoplasts. ATMYB2 activated the transcription of a reporter gene from the MYB-binding site in a sequence-specific manner. Deletion of the C-terminal region of ATMYB2 reduced the trans-activation of the reporter gene, indicating that the acidic region at the C-terminus of ATMYB2 is required for transcriptional activation. The domain exchange analysis with the yeast GAL4 revealed that the C-terminal acidic region of ATMYB2 contains a sufficient domain for trans-activation. These results indicate that ATMYB2 acts as a transcriptional activator and that the C-terminal acidic region of ATMYB2 can function as a transcriptional activation domain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / cytology
  • Arabidopsis / genetics*
  • Arabidopsis Proteins*
  • Disasters
  • Gene Expression Regulation, Plant*
  • Genes, Reporter
  • Plant Proteins / genetics*
  • Protoplasts
  • Structure-Activity Relationship
  • Trans-Activators / genetics*
  • Transcription, Genetic
  • Transcriptional Activation
  • Water / metabolism*


  • Arabidopsis Proteins
  • MYB2 protein, Arabidopsis
  • Plant Proteins
  • Trans-Activators
  • Water