Rapid and differential detection of two analogous enterotoxins of Vibrio cholerae and enterotoxigenic Escherichia coli by a modified enzyme-linked immunosorbent assay

FEMS Immunol Med Microbiol. 1997 Jan;17(1):21-5. doi: 10.1111/j.1574-695X.1997.tb00992.x.

Abstract

The principle of a novel ELISA (nylon-slip immuno-test, NSIT) was applied to the differential detection of two analogous enterotoxins, cholera toxin (CT) of Vibrio cholerae and heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli. The results obtained for CT and LT detection by a single test were sufficiently sensitive (87.9 and 100%) and specific (100 and 94.7%) in the differential detection test, when compared with the result of a colony hybridization test with DNA probes. The results suggest that the novel ELISA is applicable to the diagnosis of bacterial infections, by means of differential immunological detection of toxins in a single test.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Toxins / analysis*
  • Cholera / diagnosis
  • Cholera Toxin / analysis*
  • Cross Reactions
  • DNA / analysis
  • Diagnosis, Differential
  • Enterotoxins / analysis*
  • Enzyme-Linked Immunosorbent Assay
  • Escherichia coli / immunology*
  • Escherichia coli Infections / diagnosis
  • Escherichia coli Proteins*
  • Nucleic Acid Hybridization
  • Sensitivity and Specificity
  • Vibrio cholerae / immunology*

Substances

  • Bacterial Toxins
  • Enterotoxins
  • Escherichia coli Proteins
  • DNA
  • Cholera Toxin
  • heat-labile enterotoxin, E coli