Tissue-specific subunit of the mouse cytosolic chaperonin-containing TCP-1

FEBS Lett. 1997 Jan 27;402(1):53-6. doi: 10.1016/s0014-5793(96)01501-3.


We have cloned a novel Tcp-1-related mouse testis cDNA encoding a polypeptide of 531 amino acids which shares 81.2% identity with the zeta subunit of the mouse cytosolic chaperonin-containing TCP-1 (CCT). Immunoblot analysis of mouse testis CCT subunits separated by 2-dimensional gel electrophoresis indicates that this novel gene, Cctz-2, encodes a CCT subunit of Mr 57 000 and pI 7.1. Cctz-2 mRNA is detected only in testis whereas the other Cctz gene, Cctz-1, is expressed in all tissues investigated. The CCTzeta-2 subunit may have specific functions in the folding of testicular proteins and for interactions with testicular molecular chaperones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Chaperonin Containing TCP-1
  • Chaperonins / chemistry
  • Chaperonins / genetics*
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Humans
  • Immunoblotting
  • Isoelectric Point
  • Male
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Organ Specificity
  • Testis / metabolism*


  • CCT6B protein, human
  • DNA, Complementary
  • Tcp1 protein, mouse
  • Chaperonin Containing TCP-1
  • Chaperonins

Associated data

  • GENBANK/Z50192