The cloning and characterization of human MyD88: a member of an IL-1 receptor related family

FEBS Lett. 1997 Jan 27;402(1):81-4. doi: 10.1016/s0014-5793(96)01506-2.

Abstract

Murine MyD88, an RNA with homology both to the interleukin-1 receptor signaling domain and to 'death-domains', is rapidly upregulated during differentiation of the myeloleukemic cell line M1. We have cloned the human homologue of murine MyD88 and re-evaluated the murine sequence. The open reading frame for both species encodes a 296 amino acid protein, which for murine MyD88 is 53 amino acids longer than originally published. Human MyD88 cDNA is encoded by 5 exons, and maps to chromosome 3p21.3-p22 by fluorescence in situ hybridization (FISH). Overexpression of the death domain region leads to transcriptional activation of the IL-8 promoter.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Antigens, Differentiation*
  • Base Sequence
  • COS Cells
  • Chromosome Mapping
  • Chromosomes, Human, Pair 3
  • DNA, Complementary / genetics
  • Exons
  • Gene Expression
  • Humans
  • In Situ Hybridization, Fluorescence
  • Interleukin-8 / genetics
  • Introns
  • Mice
  • Molecular Sequence Data
  • Myeloid Differentiation Factor 88
  • Oligonucleotides, Antisense
  • Promoter Regions, Genetic
  • Proteins / chemistry
  • Proteins / genetics*
  • RNA, Messenger / genetics
  • Receptors, Immunologic*
  • Receptors, Interleukin-1 / chemistry
  • Receptors, Interleukin-1 / genetics
  • Transfection

Substances

  • Adaptor Proteins, Signal Transducing
  • Antigens, Differentiation
  • DNA, Complementary
  • Interleukin-8
  • MYD88 protein, human
  • Myd88 protein, mouse
  • Myeloid Differentiation Factor 88
  • Oligonucleotides, Antisense
  • Proteins
  • RNA, Messenger
  • Receptors, Immunologic
  • Receptors, Interleukin-1

Associated data

  • GENBANK/U84408
  • GENBANK/U84409