Abstract
Murine MyD88, an RNA with homology both to the interleukin-1 receptor signaling domain and to 'death-domains', is rapidly upregulated during differentiation of the myeloleukemic cell line M1. We have cloned the human homologue of murine MyD88 and re-evaluated the murine sequence. The open reading frame for both species encodes a 296 amino acid protein, which for murine MyD88 is 53 amino acids longer than originally published. Human MyD88 cDNA is encoded by 5 exons, and maps to chromosome 3p21.3-p22 by fluorescence in situ hybridization (FISH). Overexpression of the death domain region leads to transcriptional activation of the IL-8 promoter.
MeSH terms
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Adaptor Proteins, Signal Transducing
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Amino Acid Sequence
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Animals
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Antigens, Differentiation*
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Base Sequence
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COS Cells
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Chromosome Mapping
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Chromosomes, Human, Pair 3
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DNA, Complementary / genetics
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Exons
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Gene Expression
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Humans
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In Situ Hybridization, Fluorescence
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Interleukin-8 / genetics
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Introns
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Mice
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Molecular Sequence Data
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Myeloid Differentiation Factor 88
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Oligonucleotides, Antisense
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Promoter Regions, Genetic
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Proteins / chemistry
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Proteins / genetics*
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RNA, Messenger / genetics
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Receptors, Immunologic*
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Receptors, Interleukin-1 / chemistry
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Receptors, Interleukin-1 / genetics
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Transfection
Substances
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Adaptor Proteins, Signal Transducing
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Antigens, Differentiation
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DNA, Complementary
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Interleukin-8
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MYD88 protein, human
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Myd88 protein, mouse
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Myeloid Differentiation Factor 88
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Oligonucleotides, Antisense
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Proteins
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RNA, Messenger
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Receptors, Immunologic
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Receptors, Interleukin-1
Associated data
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GENBANK/U84408
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GENBANK/U84409