Cloning of the human IL-13R alpha1 chain and reconstitution with the IL4R alpha of a functional IL-4/IL-13 receptor complex

FEBS Lett. 1997 Jan 20;401(2-3):163-6. doi: 10.1016/s0014-5793(96)01462-7.


The human homologue of the recently cloned murine IL-13 binding protein (IL-13R alpha1) was cloned from a cDNA library derived from the carcinoma cell line CAKI-1. The cloned cDNA encodes a 427 amino acid protein with two consensus patterns characteristic of the hematopoietic cytokine receptor family and a short cytoplasmic tail. The human protein is 74% identical to the murine IL-13R alpha1, and 27% identical to the human IL-13R alpha2. CHO cells expressing recombinant hIL-13R alpha1 specifically bind IL-13 (Kd approximately 4 nM) but not IL-4. Co-expression of the cloned cDNA with that of IL-4R alpha resulted in a receptor complex that displayed high affinity for IL-13 (Kd approximately 30 pM), and that allowed cross-competition of IL-13 and IL-4. Electrophoretic mobility shift assay showed that IL-13 and IL-4 were able to activate Stat6 in cells expressing both IL-4R alpha and IL-13R alpha1, while no activation was observed in cells expressing either one or the other alone.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, CD / genetics*
  • Antigens, CD / metabolism
  • CHO Cells
  • Cloning, Molecular
  • Cricetinae
  • Gene Expression Regulation
  • Humans
  • Interleukin-13 Receptor alpha1 Subunit
  • Molecular Sequence Data
  • Receptors, Interleukin / genetics*
  • Receptors, Interleukin / metabolism
  • Receptors, Interleukin-13
  • Receptors, Interleukin-4
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • STAT6 Transcription Factor
  • Signal Transduction
  • Trans-Activators / metabolism
  • Tumor Cells, Cultured


  • Antigens, CD
  • IL13RA1 protein, human
  • Interleukin-13 Receptor alpha1 Subunit
  • Receptors, Interleukin
  • Receptors, Interleukin-13
  • Receptors, Interleukin-4
  • Recombinant Fusion Proteins
  • STAT6 Transcription Factor
  • STAT6 protein, human
  • Trans-Activators

Associated data

  • GENBANK/Y09328