Ozone depletes tocopherols and tocotrienols topically applied to murine skin

FEBS Lett. 1997 Jan 20;401(2-3):167-70. doi: 10.1016/s0014-5793(96)01463-9.


To evaluate ozone damage to hairless mouse skin, two parameters of oxidative damage, vitamin E depletion and malondialdehyde (MDA) production, were measured in vitamin E-enriched and in control skin from mice exposed to ozone (10 ppm). A 5% vitamin E solution (tocotrienol-rich fraction, TRF) in polyethylene glycol (PEG) was applied to 2 sites on the back of hairless mice, PEG to 2 sites. After 2 h, the sites were washed, one of each pair of sites covered and the mice exposed ozone for 2 h. Ozone exposure (compared with covered sites) increased epidermal MDA in PEG-treated sites, while vitamin E was unchanged. In contrast, ozone exposure significantly depleted vitamin E in TRF-treated sites, while significant MDA accumulation was prevented. This is the first demonstration that ozone exposure causes damage to cutaneous lipids, an effect which can be attenuated by vitamin E application.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Administration, Topical
  • Animals
  • Antioxidants / metabolism
  • Female
  • Lipid Metabolism
  • Lipid Peroxidation
  • Mice
  • Mice, Hairless
  • Oxidants, Photochemical / toxicity*
  • Ozone / toxicity*
  • Palm Oil
  • Plant Oils
  • Skin / drug effects*
  • Skin / metabolism
  • Vitamin E / analogs & derivatives*
  • Vitamin E / metabolism
  • Vitamin E / pharmacology


  • Antioxidants
  • Oxidants, Photochemical
  • Plant Oils
  • Vitamin E
  • Palm Oil
  • Ozone