Identification of Chicken Bu-1 Alloantigens Using the Monoclonal Antibody AV20

Vet Immunol Immunopathol. 1996 Dec;55(1-3):225-34. doi: 10.1016/s0165-2427(96)05635-8.

Abstract

The genetically polymorphic chicken antigen Bu-1 (chB6) has been identified by alloantisera raised against RPL line 6(3) (Bu-1a) and line 7(2) (Bu-1b) birds and subsequently by monoclonal antibodies (mAbs) which identify individual alloantigens. We have produced a monoclonal antibody, AV20, which recognises a monomorphic determinant on the antigen Bu-1. AV20 identifies a marker on both bursal and peripheral B cells. Staining characteristics on bursa, spleen, thymus and peripheral blood lymphocytes are similar to those of the allotypic antibodies which identify Bu-1a and Bu-1b. However, AV20 identified B cells in partially inbred birds as well as inbred lines including line 6(1) and line 7(2), indicating that it recognises a monomorphic determinant, AV20 immunoprecipitated an antigen with a Mwr of 150 kDa under non-reducing conditions and 70-75 kDa under reducing conditions indicating it is a homodimer. Serial immunoprecipitations or bursal-cell lysates from line 6(1) or line 7(2) confirmed that AV20 recognised the same antigen as mAbs against Bu-1a and Bu-1b in the respective lines.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / chemistry*
  • Antigens, Differentiation, B-Lymphocyte / analysis*
  • Antigens, Differentiation, B-Lymphocyte / immunology*
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / metabolism
  • Bursa of Fabricius / immunology
  • Bursa of Fabricius / metabolism
  • Chickens
  • Flow Cytometry
  • Immunohistochemistry
  • Precipitin Tests / veterinary

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation, B-Lymphocyte