Differential growth of N- and S-type human neuroblastoma cells xenografted into scid mice. correlation with apoptosis

J Pathol. 1996 Dec;180(4):415-22. doi: 10.1002/(SICI)1096-9896(199612)180:4<415::AID-PATH684>3.0.CO;2-A.


This study concerns the role of apoptosis in the growth of human neuroblastomas transplanted into immunodeficient SCID mice. Human neuroblastoma cell lines may consist of one or more distinct phenotypes including the neural 'N-type' and flat substrate-adherent 'S-type'. A differential phenotype-specific proliferation was apparent among S- and N-type cell clones transplanted into SCID mice when compared with the wild-type SK-N-BE(2) cell line. This differential growth capacity of the tumours was correlated with spontaneous apoptosis. Another SK-N-BE(2)-derived cell line (TGA), displaying high levels of apoptosis upon stable transfection with the full length 'tissue' transglutaminase (tTG) cDNA, was unable to induce tumour development when xenografted into SCID mice. To support these observations, the expression of apoptosis-related genes (i.e., bcl-2, p53, and tTG) in the various neuroblastomas was also investigated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / physiology*
  • Cell Division
  • Gene Expression
  • Humans
  • Mice
  • Mice, SCID
  • Mitotic Index
  • Neoplasm Transplantation
  • Neuroblastoma / metabolism
  • Neuroblastoma / pathology*
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • Transglutaminases / metabolism
  • Transplantation, Heterologous
  • Tumor Cells, Cultured
  • Tumor Suppressor Protein p53 / metabolism


  • Proto-Oncogene Proteins c-bcl-2
  • Tumor Suppressor Protein p53
  • Transglutaminases