Fluorescence detection of specific sequence of nucleic acids by oxazole yellow-linked oligonucleotides. Homogeneous quantitative monitoring of in vitro transcription

Nucleic Acids Res. 1996 Dec 15;24(24):4992-7. doi: 10.1093/nar/24.24.4992.

Abstract

We have developed a fluorescent DNA probe, oxazole yellow (YO)-linked oligonucleotide complementary to a target DNA/RNA, which can enhance the fluorescence on hybridizing with a target nucleotide. We demonstrated the applicability of the YO-linked oligonucleotide probe to real-time monitoring of the in vitro transcription process of a plasmid DNA constructed containing the 5'-terminus non-coded region of hepatitis C virus RNA. In the process of in vitro transcription in the presence of YO-linked complementary oligonucleotide, the fluorescence of the reaction mixture showed a time-dependent linear increase corresponding to the generated target RNA product.

MeSH terms

  • Benzoxazoles / chemistry*
  • Binding Sites
  • DNA Probes*
  • Fluorescent Dyes*
  • Nucleic Acids / chemistry
  • Nucleic Acids / genetics*
  • Quinolines / chemistry*
  • Sequence Analysis, DNA
  • Transcription, Genetic*

Substances

  • Benzoxazoles
  • DNA Probes
  • Fluorescent Dyes
  • Nucleic Acids
  • Quinolines
  • oxazole yellow