NF-Y Binds to the Inverted CCAAT Box, an Essential Element for cAMP-dependent Regulation of the Rat Fatty Acid Synthase (FAS) Gene

Gene. 1997 Jan 3;184(1):21-6. doi: 10.1016/s0378-1119(96)00568-9.


Using EMSA competition experiments together with supershifts and in vitro transcription/translation we show that the basal transcription factor NF-Y or a related factor binds to the cAMP-responsive inverted CCAAT box recently identified in the rat fatty acid synthase (FAS) gene from nucleotide -99 to -92 relative to the transcription start site of the FAS mRNA. This result indicates a putative novel role for NF-Y in the cAMP-dependent gene regulation in a small class of genes such as FAS and tryptophan hydroxylase. Since NF-Y is a constitutively produced factor, not surprisingly, no differences in the specific DNA/protein complex with the CCAAT(FAS) box and nuclear proteins from H4IIE cells treated with cAMP and/or insulin or not could be observed. This implies that NF-Y might be modified in response to cAMP or might interact with another factor whose properties are altered by cAMP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / immunology
  • Antibodies / metabolism
  • Binding, Competitive
  • CCAAT-Enhancer-Binding Proteins
  • Cyclic AMP / pharmacology*
  • DNA-Binding Proteins / metabolism*
  • Fatty Acid Synthases / genetics*
  • Gene Expression Regulation / genetics*
  • Insulin / pharmacology
  • Nuclear Proteins / metabolism
  • Promoter Regions, Genetic / genetics
  • Rats
  • Reticulocytes / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Tumor Cells, Cultured


  • Antibodies
  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins
  • Insulin
  • Nuclear Proteins
  • Transcription Factors
  • Cyclic AMP
  • Fatty Acid Synthases