Guanine nucleotide binding proteins in opioid-dependent patients

Biol Psychiatry. 1997 Jan 15;41(2):130-4. doi: 10.1016/s0006-3223(96)00216-8.


Guanine nucleotide binding (G) protein levels and functioning in the platelets of 19 methadone-maintained patients were compared to age and sex matched, normal controls. We found that in the methadone patients, G alpha s-levels were significantly higher, while the levels of G alpha i 1/2 and pertussis toxin catalyzed [32P]ADP ribosylation were significantly lower compared to control subjects in platelet membranes. We have further found that when all three of these biochemical indicators were combined in a discriminant function analysis, 79% of the methadone patients were correctly classified and 83% of the controls were correctly classified.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Diphosphate Ribose / blood
  • Adult
  • Blood Platelets / drug effects
  • Blood Platelets / metabolism
  • Cell Membrane / metabolism
  • Female
  • GTP-Binding Protein alpha Subunits, Gi-Go / blood
  • GTP-Binding Protein alpha Subunits, Gs / blood
  • GTP-Binding Proteins / blood*
  • Humans
  • Male
  • Methadone / therapeutic use*
  • Opioid-Related Disorders / blood*
  • Opioid-Related Disorders / diagnosis
  • Opioid-Related Disorders / rehabilitation
  • Reference Values
  • Sensitivity and Specificity


  • Adenosine Diphosphate Ribose
  • GTP-Binding Proteins
  • GTP-Binding Protein alpha Subunits, Gi-Go
  • GTP-Binding Protein alpha Subunits, Gs
  • Methadone