Expression of mRNA for interferon-gamma, interleukin-10, and interleukin-12 (p40) in normal gastric mucosa and in mucosa infected with Helicobacter pylori

Scand J Gastroenterol. 1997 Jan;32(1):22-7. doi: 10.3109/00365529709025058.

Abstract

Background: We studied the mRNA expressions of interferon-gamma (IFN-gamma), interleukin-10 (IL-10), and IL-12 in gastric biopsy and blood samples from patients with and without Helicobacter pylori infection, by reverse-transcription polymerase chain reaction (RT-PCR).

Methods: RT-PCR was performed on total RNA preparations, and the expressed mRNA were semiquantitated on the basis of band intensities on Southern blots.

Results: In gastric mucosa the expression of IFN-gamma and IL-10 was found in most patients with and without H. pylori infection, whereas IL-12 was found in most of the infected ones. The level of IFN-gamma and IL-10 did not differ between groups, whereas the IL-12 level was significantly higher in those with H. pylori infection. In the blood IFN-gamma expression was found in most samples, with higher level in patients with gastritis than in normals. Few blood samples (33%) had IL-12, and none had IL-10.

Conclusion: IFN-gamma and IL-10 expressions in healthy mucosa may indicate a biologic role in a healthy state. IL-12 expression in mucosa was related to the presence of bacterial stimulant and therefore resembles proinflammatory cytokines.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adult
  • Aged
  • Blotting, Southern
  • Gastric Mucosa / metabolism*
  • Gastritis / immunology*
  • Gastritis / microbiology
  • Gastritis / pathology
  • Helicobacter Infections / immunology*
  • Helicobacter Infections / microbiology
  • Helicobacter Infections / pathology
  • Helicobacter pylori* / isolation & purification
  • Humans
  • Interferon-gamma / genetics*
  • Interleukin-10 / genetics*
  • Interleukin-12 / genetics*
  • Middle Aged
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Messenger / metabolism*
  • RNA-Directed DNA Polymerase

Substances

  • RNA, Messenger
  • Interleukin-10
  • Interleukin-12
  • Interferon-gamma
  • RNA-Directed DNA Polymerase

Associated data

  • GENBANK/M65290