Vasopressin-stimulated Ca2+ reabsorption in rabbit cortical collecting system: effects on cAMP and cytosolic Ca2+

Pflugers Arch. Nov-Dec 1996;433(1-2):109-15. doi: 10.1007/s004240050255.


The effect of arginine vasopressin (AVP) on transepithelial Ca2+ transport in primary cultures of rabbit cortical collecting system cells was examined. Addition of AVP to the basolateral side of the monolayer dose-dependently (EC50 = 0.7 nM) increased active Ca2+ reabsorption from a basal value of 85 +/- 2 to a maximum value of 124 +/- 3 This was paralleled by a dose-dependent (EC50 = 1.1 nM) increase in cellular adenosine 3', 5'-cyclic monophosphate (cAMP) content. Both effects of AVP were mimicked by the V2 agonist deamino-Cys,D-Arg8-vasopressin (dDAVP) and forskolin. Addition of either AVP or dDAVP to the basolateral side evoked a sustained increase in cytosolic free Ca2+ concentration, which resulted from both Ca2+ entry and release from internal stores. Only the effect on Ca2+ entry was mimicked by forskolin, demonstrating that cAMP acts by activating a Ca2+ influx pathway. The present findings demonstrate that AVP stimulates transcellular Ca2+ transport in the cortical collecting system through activation of basolateral V2 receptors coupled to adenylyl cyclase to increase the cellular cAMP content.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorption / drug effects
  • Animals
  • Arginine Vasopressin / pharmacology*
  • Calcium / metabolism*
  • Cells, Cultured
  • Cyclic AMP / metabolism*
  • Cytosol / metabolism*
  • Epithelial Cells
  • Epithelium / metabolism
  • Kidney Cortex
  • Kidney Tubules, Collecting / cytology
  • Kidney Tubules, Collecting / drug effects
  • Kidney Tubules, Collecting / metabolism*
  • Rabbits


  • Arginine Vasopressin
  • Cyclic AMP
  • Calcium