The effect of TGF-beta 1 on cell proliferation and proteoglycan production in human melanoma cells depends on the degree of cell differentiation

Cancer Lett. 1996 Dec 3;109(1-2):39-47. doi: 10.1016/s0304-3835(96)04402-3.


The effect of transforming growth factor beta 1 (TGF-beta 1) on cell proliferation, colony formation in soft agar and synthesis and structure of proteoglycans was studied in three human melanoma cell lines at different stages of differentiation: SK-mel-1.36-1-5 (early), SK-mel-3.44 (intermediate) and SK-mel-23 (late). TGF-beta 1 potently inhibited cell growth in monolayer as well as in soft agar. TGF-beta 1 increased the release of sulfated proteoglycans into the medium, including the cell-specific melanoma proteoglycan, mel-PG, and induced changes in disaccharide composition and sulfation of the glycosaminoglycan chains. In all the cases, the effect of TGF-beta 1 was more pronounced in the most undifferentiated cell line SK-mel-1.36-1-5 than in the SK-mel-3.44, whereas it had no effect on the most differentiated SK-mel-23 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation
  • Cell Division / drug effects
  • Humans
  • Melanoma / metabolism*
  • Melanoma / pathology*
  • Neoplasm Proteins / metabolism*
  • Proteoglycans / metabolism*
  • Skin Neoplasms / metabolism*
  • Skin Neoplasms / pathology*
  • Transforming Growth Factor beta / pharmacology*
  • Tumor Cells, Cultured / drug effects
  • Tumor Stem Cell Assay


  • Neoplasm Proteins
  • Proteoglycans
  • Transforming Growth Factor beta